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Figure 8


Fig. 8. Inhibition of p53-sensitized SiHa cells to H2O2-induced oxidative damage. (A) 48 hours after transfection with negative control plasmid (SiHa/N) or p53 shRNA plasmid (SiHa/sip53), cells were treated with various concentrations of H2O2 for either 12 hours or 24 hours, after which time cell viability was measured with an XTT assay. Results shown are expressed as mean ± s.d. of triplicate microcultures. *P<0.01 compared with the corresponding negative control using Student's t-test. (B) Apoptosis and death levels of SiHa/N and SiHa/sip53 cells 12 hours after treatment with 0.1, 0.5 and 1 mM of H2O2 as detected by FACS after Annexin V/PI staining. Apoptotic (Annexin V+/PI–), dead (Annexin V+/PI+) and alive (Annexin V–/PI–) populations were readily identified. The rates of apoptotis and death are shown in upper right and lower right panels, respectively. *P<0.01 compared with the corresponding untreated cells (Student's t-test).





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