spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Reduced aPKC{lambda} activity results in increased cell death in PAR6{Delta}N cells. (A) aPKC{lambda} activity and PAR6 expression levels were analyzed in wild-type MDCK and PAR6{Delta}N cells by immunoblot using, respectively, phospho-aPKC{lambda}, total-aPKC{lambda} and PAR6B antibodies. The quantification of three independent experiments is depicted. *P<0.05. (B) MDCK cysts grown in collagen were treated with either the non-myristoylated (control) or myristoylated form of aPKC{zeta} pseudosubstrate peptide, at the indicated concentrations, at day 4; aPKC{zeta} pseudosubstrate-containing media were refreshed at day 6. Immunostaining with Ki-67 (green) and cleaved caspase-3 (red) were performed at day 7. Bar, 10 µm. (C) Percentage of cysts containing apoptotic cells was quantified by cleaved caspase-3 staining comparing control and aPKC inhibitor-treated cells. Results are the mean±s.d. of three experiments.





Right arrow Return to article