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Figure 2


Fig. 2. Chemotaxis of temperature-sensitive NSF cells. Control Ax2 and temperature sensitive NSF cells (strain HM1067, nsfA2) were made aggregation-competent at 20°C, then shifted to the restrictive temperature of 28°C for at least 20 minutes, before being filmed moving towards a micropipette containing cAMP. The left-hand panels show stills from the films (Movies 1 and 2 in supplementary material) taken after 20-30 minutes. The insets show a typical elongated chemotaxing Ax2 cell and a rare nsfA2 cell that is chemotaxing efficiently but maintains a smooth profile and is only moderately elongated. The movement speeds of 68 (Ax2) or 80 (nsfA2) randomly chosen cells from each condition are tabulated in the right hand panels. Bar, 20 µm.





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