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Fig. 7. Surface area and volume of a cell moving under agarose. Ax2 cells expressing cAR1-GFP were induced to chemotax under 0.6% agarose towards cAMP. Bursts of three sets of confocal stacks (12 sections taking three seconds) were taken at approximately 25 seconds intervals and the areas and volumes obtained from each burst treated as repeat measurements at the same time point, thus allowing statistical analysis (1-way ANOVA, with Tukey's test). (A) Relative surface area and volume of the cell body (without filopodia); (B) number and total length of filopodia, measured manually; (C) relative surface area of cell body and filopodia (assumed to be 0.1 µm in diameter); plotted to the same scale, but note the different origins. The increase in surface area at 0-69 seconds and the subsequent decline at 69-174 seconds are both significant at P<0.001, whereas none of the volume changes are (P>0.05). The cell is the same one as shown in Fig. 6 with an initial surface area of the body of 698 µm² and of the filopodia of 3.5 µm² (0.5 % of the total surface area); and a volume of 586 µm³.

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