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Fig. 2. Expression of the NFAT2 protein isoform in human neutrophils. (A) The potential presence of the five NFAT isoforms was analysed by western blotting of whole-cell extracts from unstimulated neutrophils obtained immediately after their isolation from both human healthy donors (N1-N3) and human allergic patients (N4-N6), as well as from PBLs (L) and Jurkat T cells (J) stimulated with 100 nM plus 500 nM ionomycin used as positive controls. 
-actin was used as an internal control to verify that equal amounts of protein were loaded per lane. All blots shown are representative of at least three independent experiments in each group of individuals, with similar results. (B) Flow cytometry analysis of NFAT2 expression in neutrophils isolated from an allergic patient (left panel) and a healthy donor (right panel). After isolation, two-colour flow cytometry analysis of CD66b and NFAT2 expression was performed. Results obtained are expressed as the percentage of double-positive (CD66b+ and NFAT2+) cells. Results shown are representative of at least three independent experiments in each different group of individuals.
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