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Fig. 3. Anti-IgE-elicited NFAT2 nuclear translocation in human neutrophils. Constitutive NFAT2 expression in human neutrophils. (A) Neutrophils isolated from an allergic patient were stimulated with 10 µg/ml anti-IgE (
-IgE) for the indicated times (up to 6 hours), or incubated with 10 µg/ml non-specific goat IgG (IgG) for 4 hours. Then, nuclear and cytosolic extracts were obtained. (B) Whole-cell extracts were obtained immediately after neutrophil isolation from an allergic patient (basal), or neutrophils were cultured in the absence or presence of 10 µg/ml anti-IgE (-IgE) for the indicated times (up to 24 hours), and then whole-cell extracts were obtained. Cellular, cytosolic and nuclear NFAT2 levels were analysed by western blotting, using anti-human NFAT2-specific Abs. 
-actin was used as an internal control. The blot shown is representative of three independent experiments with similar results. Histograms above each lane show the mean (± s.e.m.) values quantified from the blots from three separate experiments.
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