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Figure 4


Fig. 4. Anti-Fc{epsilon}Rs- and antigen-elicited NFAT2 nuclear translocation in human neutrophils. Neutrophils from three allergic patients sensitised to either T9 (A), E1 (B) or W6 (C) were stimulated for 4 hours with 10 µg/ml anti-IgE ({alpha}-IgE) or with the indicated specific antigens (W6, D1, D2, G3, T9, E1 or E2) at 10 µg/ml. (D) Neutrophils from an allergic patient were incubated with 5 µg/ml anti-Fc{epsilon}RII ({alpha}-Fc{epsilon}RII), anti-Mac-2 ({alpha}-Mac-2), or anti-Fc{epsilon}RI ({alpha}-Fc{epsilon}RI) Abs or mouse IgG1 for 4 hours at 37°C. Then, nuclear extracts were obtained, and NFAT2 levels were analysed by western blotting, using anti-human NFAT2-specific Abs. Equal amounts of protein were loaded per lane. Histograms above each lane show the mean (± s.e.m.) values quantified from the blots from three separate experiments.





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