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Fig. 7. Anti-IgE-elicited NFAT2 DNA-binding activity in human neutrophils. Role of CaN. (A,B) Neutrophils from an allergic patient were treated either with 10 µg/ml anti-IgE (-IgE) for the indicated times (A), or with the indicated doses of anti-IgE for 4 hours (B). (C) Neutrophils from an allergic patient sensitised to G₃ were pretreated with 1 µg/ml CsA or 50 µg/ml VIVIT peptide for 1 hour, and then treated with 10 µg/ml anti-IgE (-IgE) or 10 µg/ml G₃ antigen for 4 hours. Nuclear extracts were then obtained, and binding to the ³²P-labelled NFAT oligonucleotide probe was assessed by electrophoretic mobility shift assays. Incubation of neutrophils with 10 µg/ml non-specific IgG for 4 hours was also carried out as a negative control (IgG). Assays performed in the presence of 1 µl of anti-NFAT2 (Ab) or carrying a 100-fold molar excess of unlabelled oligonucleotide (^*) are also shown.

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