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Figure 2


Fig. 2. The lvsB mutant cells contain fewer H+-ATPase-negative post lysosomes. (A) Lysosomes (p80-positive, H+-ATPase-positive; arrowheads) and post lysosomes (p80-positive, H+-ATPase-negative; arrows) were identified by double immunofluorescence. The compartments positive for H+-ATPase but negative for p80 represent contractile vacuoles (pinhead). Bars, 5 µm. (B,C) A decreased number of post lysosomes was found in lvsB mutant cells. To determine the number of post lysosomes (defined as p80-positive and H+-ATPase-negative vacuoles), cells were scanned from bottom (attached to the substratum) to top, and post lysosomes were counted. In B, the percentage of cells with a given number of post lysosomes is indicated. (D) The size of post lysosomes was similar in mutant and WT cells. All experiments were repeated three times with equivalent results. One representative set of data is shown in B. Means + s.e.m. are shown in C and D.





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