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Figure 4


Fig. 4. Endosomal H+-ATPase and actin are mutually exclusive. (A) Actin was present only on vacuoles stained for p80 but not for H+-ATPase (post lysosomes, arrows). Conversely, actin was not observed on vacuoles positive for both p80 and H+-ATPase (lysosomes, arrowheads). Bar, 5 µm. (B) Percentage of the p80-positive, actin-positive vacuoles containing no H+-ATPase. The means + s.e.m. of three independent experiments are indicated. All the p80-positive, actin-positive vacuoles of at least 30 cells were analyzed for each cell type in each experiment.





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