JCS006007 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Chromosomal distribution of SUUR binding. Each vertical line represents the average log of SUUR∼Dam versus Dam methylation ratio of a probed gene at its chromosomal position (in megabases). C, centromere; T, telomere. Width of the vertical lines indicates the size of the probed regions. Red lines indicate statistically significant SUUR target genes and black lines indicate SUUR non-targets. Gray lines indicate standard deviations.

• Supplemental Figure S2 -

  Fig. S2. SUUR binds gene clusters. Bar graph showing the number of SUUR gene clusters encompassing the indicated number of genes.

• Supplemental Figure S3 -

  Fig. S3. Chromosomal distribution of HP1 binding. Each vertical line represents the average log of Dam-HP1 versus Dam methylation ratio of a probed gene at its chromosomal position (in megabases). C, centromere; T, telomere.. Width of the vertical lines indicates the size of the probed regions. Red lines indicate statistically significant HP1 target genes and black lines indicate HP1 nontargets. Gray lines indicate standard deviations.

• Supplemental Figure S4 -

  Fig. S4. SUUR shares target genes with HP1 and SU(VAR)3-9. Venn diagram of the overlap between targets of SUUR, HP1 and SU(VAR)3-9. Information was available for 5613 probes. Dataset of SU(VAR)3-9 was taken from Greil et al. (Greil et al., 2003).

• Supplemental Figure S5 -

  Fig. S5. Many of SUUR target genes are flanked by repeats. Bivariate scatter plot of log-transformed data comparing SUUR and HP1 profiles. Targets for either or both proteins, SUUR and HP1, are depicted as filled dots. Targets flanked by more than 5% repeats within the vicinity of 20 kb are colored red, targets flanked by less than 5% repeats are colored blue.

• Supplemental Figure S6 -

  Fig. S6. SUUR is associated with distinct subsets of Pc, HP1 and Lam targets. Venn diagrams show the overlap between targets of SUUR, Pc, Lam (A), SUUR, HP1, Lam (B) and HP1, Pc, Lam (C). Number of investigated probes is indicated at the bottom right corner of each diagram. Datasets of Pc and Lam were taken from Tolhuis et al. and Pickersgill et al., respectively (Pickersgill et al., 2006; Tolhuis et al., 2006).

• Supplemental Figure S7 -

  Fig. S7. Cluster analysis of the developmental expression time course of genes that are SUUR targets in Kc cells. E, embryos; L, larvae; M, metamorphosis; Am, adult male; Af, adult female. All gene expression data were taken from Arbeitman et al. (Arbeitman et al., 2002).