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Fig. 5. Similar phenotypes between Par-3- and afadin-knockdown MDCK cells. (A) Reduction in the immunofluorescence signals for claudin-1, JAM-A and ZO-1, as well as those for E-cadherin and afadin, but not those for p120^ctn, -catenin, and -catenin, at cell-cell adhesion sites in Par-3-knockdown MDCK cells. After Ca²⁺ switch, the cells were fixed and stained with various combinations of the indicated Abs. Bars, 10 µm. (B) Re-concentration of the immunofluorescence signals for claudin-1, JAM-A and ZO-1, as well as that for afadin at cell-cell adhesion sites in the Par-3-knockdown MDCK cells that re-expressed GFP–Par-3. After Ca²⁺ switch, the cells were fixed and stained with various combinations of the indicated Abs. Bars, 10 µm.

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