spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 8


Fig. 8. The CC domain and PR region are important for cell migration. (A) Podocytes were transfected with two different concentrations of CMS siRNA or control siRNA in triplicate. After 48 hours cells were subjected to serum-stimulated Boyden chamber migration assay. Cell lysates were analyzed by western blotting for CMS and actin. (B) Podocytes expressing mutated forms of CMS display reduced migration. Cells stably expressing CMS, CMS {Delta}CC, CMS {Delta}PR or vector control were subjected to a serum-stimulated migration assay for 4 hours. Data represent the mean ± s.d. of triplicates from three independent experiments (n=3; right panel; *P<0.01; **P=0.056). (C) Transformed NIH 3T3 cells display reduced migration. Src*3T3 cells expressing the indicated peptides were subjected to a serum-stimulated migration assay for 2 hours. Data represent the mean ± s.d. of triplicates from three independent experiments (n=3; *P<0.0001; **P<0.002). P values were calculated using Student's t test. Protein expression was determined by western blot analysis.





Right arrow Return to article