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Figure 10


Fig. 10. Retromer is required for endosome-to-TGN retrieval but AP-1 is not. (A) siRNA knockdown (KD) of VPS26 or µ1A is assessed by western blotting lysates from KD cells. Loss of VPS26 results in degradation of VPS35, whereas loss of µ1A causes the beta1 subunit to be degraded. The transferrin receptor (TfnR) and actin are unaffected by the siRNA knockdowns and serve as loading controls in this experiment. (B) Control or VPS26 or µ1A KD cells were analysed by antibody uptake. Loss of VPS26 results in a block in endosome-to-TGN retrieval of the CD8-CIMPR but loss of µ1A does not cause the same phenotype. (C) The CD8-CIMPR accumulates in a Snx1-positive endosome (indicated by arrows) in VPS26 KD cells, but not in µ1A KD cells. Loss of either VPS26 or µ1A resulted in cells becoming enlarged and multinucleated. Bar, 20 µm.





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