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Figure 2


Fig. 2. Alignment of CIMPR tails and schematic diagram of the truncations of the CIMPR tail. (A) The cytoplasmic tail sequences of the CIMPR from species ranging from zebrafish through chicken, marsupials, rodents, primates and humans were aligned using the ClustalW program (http://www.ch.embnet.org/). The sequences were aligned according to homology and then the shading added using BOXSHADE (http://www.ch.embnet.org/). The numbering of the residues applies to the cow sequence which was used to make the CD8-CIMPR reporter and all of the various truncations with the exception of the {Delta}1-19{Delta}LL construct, which was based upon the rat CIMPR tail sequence. The YXX{Phi} and SDEDLL motifs and other regions known to bind sorting/trafficking machinery such as AP-1 are highlighted by the black bars. The alignment reveals that the most conserved region of the CIMPR tail lies in the first 100 amino acids. (B) Schematic representation of the different CD8-CIMPR truncations. The locations of the YXX{Phi} and SDEDLL motifs are again indicated.





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