spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. The {Delta}1-75 truncation accumulates in VPS26-positive endosomes and is unstable. (A) Cells expressing the {Delta}1-75 truncation were incubated for three hours continuously with the anti-CD8 antibody prior to fixation. Cells were labelled with anti-VPS26 antiserum followed by appropriate secondary antibodies. There is significant colocalisation of the endocytosed anti-CD8 and VPS26 (shown enlarged in the inset boxes and indicated by arrows). Bar, 20 µm. (B) Cells expressing the CD8-CIMPR and truncations were treated with 100 µg/ml cycloheximide for 0, 3 or 6 hours. Cells were then lysed and the CD8 reporter immunoprecipitated and subjected to SDS-PAGE and western blotting. The {Delta}1-75 truncation is unstable and undetectable after 3 hours of cycloheximide treatment.





Right arrow Return to article