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Figure 9


Fig. 9. The WLM motif is necessary for the interaction with retromer. (A) Cells expressing CD8-CIMPR and various truncations were lysed and the CD8 reporter was immunoprecipitated under native conditions. The immunprecipitates were analysed by SDS-PAGE and western blotting. Retromer and AP-1 was able to co-immunoprecipitate with CD8-CIMPR, {Delta}75-163 and {Delta}56-143 but not {Delta}1-75. (B) Similar to A, cells expressing CD8 reporter were analysed by native immunoprecipitation. The CD8-WLM-AAA mutant, which has an almost full-length tail and localises to endosomes, does not co-immunoprecipitate with retromer or AP-1. (C) CD8-CIMPR cells were fixed and labelled with antibodies against VPS26 and AP-1. There is no obvious colocalisation present. Bar, 20 µm.





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