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Figure 2


Fig. 2. Analysis of cell shape in rot1 strains. Exponentially growing cultures of the wild-type (W303), tetO7:ROT1 (MCY68), clb2 (CD104-2c) and clb2 tetO7:ROT1 (JCY255) strains were synchronized by {alpha}-factor arrest in the presence of 5 µg/ml doxycycline. After release, the bud length/bud width ratio in anaphase and telophase cells (as deduced by the presence of segregated nuclei after DAPI staining of DNA) was measured. Values are the mean+s.d. of three experiments. Images of representative cells for each strain are shown.





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