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Figure 4


Fig. 4. Connection between Rot1 and the cell integrity pathway. (A) Tenfold serial dilutions of exponentially growing cultures of the wild-type (W303, upper panel; 1783, middle panel; and OHNY1, lower panel), tetO7:ROT1 (MCY68, upper panel; MCY186, middle panel; and MCY200, lower panel), pkc1 (JC6-3a), tetO7:ROT1 pkc1 (JCY433), slt2 (DL454), tetO7:ROT1 slt2 (JCY431), rho1 (HNY21) and tetO7:ROT1 rho1 (MCY158) strains were spotted onto YPD medium with or without 0.5 µg/ml doxycycline and incubated at 28°C for 3 days. (B) Western analysis of the phosphorylation of Slt2 in extracts from wild-type (CML240) and tetO7:ROT1 (JCY216) cells grown on YPD and incubated in the presence of 5 µg/ml doxycycline for 8 hours. A loading control of total protein is shown (* lower panel).





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