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Figure 5


Fig. 5. Organization of the actin cytoskeleton in the tetO7:ROT1 mutant strain. (A) Exponentially growing cells of the wild-type (CML240) and the tetO7:ROT1 (JCY216) strains were incubated in the presence of doxycycline for 8 hours and fixed. Pictures show DIC images, DAPI staining of DNA and Alexa Fluor 498-labeled phalloidin staining of F-actin. (B) Wild-type (CML240) and tetO7:ROT1 (JCY216) cells were synchronized by addition of {alpha}-factor in the presence of 5 µg/ml doxycycline. After release, cells at the different cell cycle stages were analyzed as described above.





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