spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 9


Fig. 9. Genetic interactions between the ROT1 gene and genes of the ubiquitin-proteasome pathway. (A) Tenfold serial dilutions from exponentially growing cultures of the wild type (W303 or WCG4{alpha} in the upper panel), tetO7:ROT1 (MCY68 or MCY125 in the upper panel), pre1 pre2 (WCG4-11/22), pre1 pre2 tetO7:ROT1 (MCY32), cdc16, cdc16 tetO7:ROT1 (JCY553), apc2 (KTM200U) and apc2 tetO7:ROT1 (JCY555) were spotted onto YPD medium with or without 0.5 µg/ml doxycycline (Dox) and incubated at 30° for 3 days. (B) Tenfold serial dilutions from exponentially growing cultures of the cdc20 and cdc20 tetO7:ROT1 (MCY139) strains transformed with a centromeric plasmid containing the ROT1 gene as indicated, were spotted onto YPD medium with or without 5 µg/ml doxycycline and incubated at 28°C or 37°C for 3 days.





Right arrow Return to article