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Figure 3


Fig. 3. The DAX domain is required for efficient puncta formation. (A-C) COS-7 cells expressing wt or mutant axin, as indicated were fixed, and (C) stained with antibody against HA (the punctate pattern of wt HA-axin is essentially the same as in A; not shown). Note that {Delta}DAX and HA-axin_M12 (a mutant version of axin, the self-association of which is expected to be blocked; see text) produce a less punctate, more diffuse pattern, indicating that the ability of axin to form puncta depends at least partly on its DAX domain. Scale bar, 15 µm.





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