spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. The recruitment of axin into Dvl2 puncta requires extensive DIX-domain flanking sequences. (A) TCF transcription assays in 293 cells, transfected with a TCF-dependent firefly luciferase reporter (SuperTOP; 50 ng) and an internal control (CMV-renilla; 25 ng), and with full-length Dvl2 (200 ng) or Dvl2 truncations as indicated (500 ng); bars indicate standard deviations. (B) Western blot, probed with anti-FLAG antibody, showing expression levels of Flag-Dvl2 constructs, as indicated. (C-F) HeLa cells, cotransfected with HA-axin (green) and full-length FLAG-Dvl2 or truncations (red), as indicated in panels, fixed and stained with antibodies against their tags; note the two sets of cytoplasmic puncta in D (red or green), but the co-localisation of Dvl and axin puncta in F (yellow), indicating that the minimal axin-recruiting sequences of Dvl2 are contained within amino acids 1-389. Scale bar, 15 µm.





Right arrow Return to article