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Fig. 8. Dvl2-axin puncta remain dynamic after Wnt-induced recruitment to the plasma membrane. (A-D) Fixed HeLa cells, co-expressing Fz8, LRP6 and GSK3
(as in Fig. 1B) in addition to (A,B) HA-Dvl2 plus axin-GFP or (C,D) Dvl2-GFP plus HA-axin, (A,C) before or (B,D) after 2 hours of exposure to recombinant Wnt3a. (E,F) Graphs of FRAP experiments conducted in HeLa cells, transfected and Wnt-stimulated as in B,D; individual membrane-associated puncta were bleached, and fluorescence recovery of (E) axin-GFP or (F) Dvl2-GFP was monitored, as in Fig. 7. Note that the recovery rates of these puncta are similar to those of cytoplasmic Dvl2-GFP puncta, and of axin-GFP recruited into cytoplasmic Dvl2 puncta, in HeLa cells (not shown) and in COS-7 cells (Fig. 7B-D).
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