spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Analysis of GFP-Kv2.1-loopBAD {Delta}C mobility on the cell surface. HEK cells expressing a GFP-Kv2.1-loopBAD channel without the C-terminal 318 amino acids ({Delta}C) were enzymatically biotinylated and tagged with streptavidin-labeled 655 quantum dots as described in the Materials and Methods. (A) Non-clustered cell surface distribution of this mutant channel in three dimensions. (B) The Qdot single-particle track used to generate the MSD plot indicated in C. In this case a diffusion coefficient of 0.10 µm2/second was calculated from the initial slope. Overall the mean diffusion coefficient equalled 0.07±0.04 µm2/second, n=10, for these mutant channels incapable of forming surface clusters. Cells were imaged every 0.55 seconds.





Right arrow Return to article