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Figure 1


Fig. 1. CDK11 RNAi causes G2/M cell-cycle accumulation, impaired proliferation and cell death. (A) Western blot analysis showing downregulation of the CDK11p110 and CDK11p58 proteins. HeLa cells were transfected with CDK11 RNAi-N (pSUPER/A461), CDK11 RNAi-C (pSUPER/A2184) or the control plasmid (pSUPER). After 72 hours, cell lysates were prepared and subjected to electrophoresis and immunoblot analysis. Immunoblots were probed with anti-CDK11 P1C antibody (Trembley et al., 2002). (B) CDK11 RNAi impaired cell proliferation. Phase-contrast photographs of HeLa cells 72 hours after transfection with CDK11 or control RNAi plasmids. Bar, 100 µm. (C) DNA content analysis 72 hours after transfection showing increased G2/M and sub-G1 fractions resulting from CDK11 RNAi. (D) Retarded cell growth caused by CDK11 RNAi. Equal numbers of cells were transfected with pSUPER (control) or pSUPER-CDK11-RNAi (pSUPER/A2184) plasmids and plated into dishes for culturing. Cells were counted 72 hours after transfection. The bars represent the mean from triplicate experiments with standard deviation.





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