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Fig. 5. CDK11 depletion leads to premature removal of centromeric cohesin complexes. HeLa cells expressing an inducible Myc-tagged human Scc1 were transfected with CDK11 RNAi or control RNAi plasmids for 48 hours with or without 6 hours of nocodazole treatment before harvesting. The cells were analyzed by immunofluorescence staining using antibodies against the Myc tag to detect Myc-human Scc1 (red) and the kinetochores CREST antigen (green). (A) Result of the analysis of 200-300 mitotic cells examined for the presence of a Myc-Scc1 signal on centromeric kinetochores in a representative experiment. (B) Representative mitotic cells. Bar, 5 µm.