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Figure 6


Fig. 6. CDK11 depletion leads to altered Sgo1 localization on the kinetochores. (A) HeLa cells were analyzed by indirect immunofluorescence 48 hours after transfection with CDK11 RNAi or control plasmids using antibodies to Sgo1 (red) and CREST (green). DNA was stained with DAPI (blue). (B) Bub1 dissociates prematurely from the kinetochores upon depletion of CDK11. HeLa cells treated with CDK11 RNAi and control RNAi were stained for Bub1 (red) and DAPI (blue) 48 hours after transfection. Bars, 5 µm.





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