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Figure 1


Fig. 1. Identification of cell-type-specific genes expressed in a particular subset of retinal neurons. (A) Schematic drawing of the screening strategy. (1) E13.5 mouse retina was dissociated into single cells; (2) they were sorted into 96-well PCR tubes using FACS; (3) cDNAs were amplified by representative RT-PCR; (4) The cell types were retrospectively identified by the expression of Math5 (ganglion cells) and Crx (cone photoreceptor cells) and subtractive PCR was carried out using cDNA derived from single retinal ganglion (#3) and cone photoreceptor (#13) cells. (B,C) Expression pattern of cell-type-specific genes with unknown function in E14 retina obtained from putative retinal ganglion cells (B) or cone photoreceptor cells (C). Note that Th9 was assumed to be expressed in retinal ganglion cells in early migratory stages. GenBank accession numbers for #0.31, #1.46, #4.79 and #7.60 are AB294516, AB294517, AB294518, and AB294519, respectively. (D) Higher magnification images of in situ hybridization of E14 retina stained for #1.46 (left) and Math5 (right). Note that the transcript of #1.46 was exclusively localized to the nucleus. Bars, 20 µm (B,C); 5 µm (D).





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