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Fig. 2. Gomafu RNA possesses mRNA-like characteristics. (A) Schematic illustration of the genomic structure of Gomafu. The region homologous to the EST clones AK053540 and AK028326 are shown in a bold line below. Arrowheads indicate positions of primer sets used to obtain overlapping cDNA. (B) Multiple tissue northern analysis of Gomafu RNA. Note that a single 9 kb band was detected only in the brain sample. (C) RNaseH northern analysis of Gomafu RNA. Note that no product greater than the predicted size was observed after RNaseH (RNH) digestion. Sizes of the full-length Gomafu RNA and RNaseH-digested fragments are indicated by filled and open arrowhead, respectively. The full-length transcript was detected in an overexposed blot, whose signal was relatively weak owing to insufficient transfer from the 1.5% agarose gel. (D) Splicing isoforms confirmed by RT-PCR. Frequency of each isoform among the cloned fragments is shown on the right. Arrowheads indicate positions of primer sets used to identify the splicing variants. GenBank accession numbers are listed on the right. (E) Northern blot analysis of poly(A)+ and total RNA derived from E14 brain. Gomafu RNA is enriched in the poly(A)+ fraction. (F) Northern blot analysis of stability of Gomafu RNA. Primary cultures of E15 brain cells were treated with 
-amanitin for indicated time. mRNA of Gomafu and 
-actin showed similar kinetics.
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