spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. NTF-dependent and NTF-independent types of axonal growth differ in their response to signal transduction inhibitors in terms of axonal morphology. (A-C) The SFK inhibitor SU6656 reduces dramatically axonal length and branching during the NGF-dependent axonal growth at 12 hours in culture. The number of branches (E,F) but not axonal length (D,F) was significantly reduced by SU6656 during axonal growth in the absence of NTFs at 40 hours in culture. The Cdk5 inhibitor roscovitine (Rosc) does not affect NGF-dependent axonal length (G) and number of branches (H) at 12 hours in culture. In contrast, roscovitine strongly suppresses axonal length (J) and branching (K) during axonal growth in the absence of NTFs at 40 hours in culture. (C,F,I,L) Distribution of the length and the number of branches of the longest axon of individual neurons. {blacktriangleup}, SU6656 treatment (C,F) and roscovitine treatment (I,L). For each condition, data are shown for 30 neurons from three independent experiments; the data in A,B,D,E,G,H,J,K are means ± s.e.m. Where indicated, NGF was applied at 100 ng/ml, SU6656 at 2 µM, roscovitine at 50 µM.





Right arrow Return to article