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Figure 6


Fig. 6. The roscovitine-sensitive outgrowth at 40 hours is not induced by endogenous NTFs. (A) Axonal outgrowth at 40 hours is not inhibited by TrkB-FC chimera (200 ng/ml), anti-NGF (250 ng/ml) and anti-GDNF (183 ng/ml) blocking antibodies, and by the glial inhibitor cytosine 1-beta-D-arabinofuranoside (1 µM) (AraC). (B) Blocking antibodies to GDNF and NGF block outgrowth in GDNF- and NGF-treated cultures, respectively. Anti-GDNF and anti-NGF antibodies were applied at the same concentration as in A. (C) Conditioned medium collected from DRG cultures at 40 hours does not activate axonal growth in newly plated neurons. All values are means ± s.e.m. of three experiments





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