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Fig. 5. pten– cells respond to the increasing temporal gradient of cAMP in the last three of a series of four temporal waves generated in a perfusion chamber in the absence of established spatial gradients. These waves mimic the temporal dynamics of natural waves (Tomchik and Devreotes, 1981), but the response is lower than that of parental AX2 cells. (A) Time plots of the instantaneous velocity (Inst. Vel.) of two representative AX2 cells responding to four successive temporal waves of cAMP. AX2 cells exhibit an increase in velocity in the front of the last three of four successive temporal waves of attractant. They do not respond to the first wave, as previously reported (Varnum et al., 1985; Wessels et al., 1992). (B) The instantaneous velocity of three representative pten– cells responding to four successive temporal waves of cAMP. pten– cells exhibit, on average, an increase in velocity in the front of the last three waves, but the magnitude of the increase is far below that of AX2 cells. Behavior in the front of each wave is color-coded blue.
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