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Fig. 9. PTEN is constitutively localized in the cortex. AX2 cells expressing GFP-PTEN were analyzed in buffer, in a spatial gradient of attractant and in the front of the third in a series of temporal waves of cAMP. (A) Perfused with buffer in a perfusion chamber in the absence of attractant. (B) Oriented up a spatial gradient of cAMP. (C,D) In the front and at the peak, respectively, of the third in a series of temporal waves of cAMP. LSCM line scans of pixel intensity are presented below each LSCM image, and the position of each line scan is indicated by a white line across each image. Note the similarity of peaks at cell edges under all conditions.
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