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Figure 6


Fig. 6. GNL3L competes with SRC1 and SRC2 for ERR{gamma} binding. Agarose-bound GST fusion proteins of ERR{gamma} (1 µg) were used to pull down whole-cell lysates containing a fixed amount of SRC1 (A) or SRC2 (B), mixed with increasing amounts of the wild-type GNL3L (A1,B1) or the dI mutant lacking the ERR{gamma}-interacting domain (A2,B2). Whole-cell proteins in each sample were adjusted to the same amount. In the agarose-retained portions (R), the interaction between GNL3L and ERR{gamma} can reduce the amount of SRC1 and SRC2 bound by ERR{gamma} in a dose-dependent manner, but the dI mutant fails to do so. Conversely, when GST-ERR{gamma} fusion proteins were used to pull down the same amount of GNL3L in the presence of increasing amounts of SRC1 (C) or SRC2 (D), SRC1 and SRC2 were able to reduce the amount of GNL3L bound by ERR{gamma} in a dose-dependent way as well. Proteins in the agarose-bound fraction and in the supernatant are indicated by (R) and (S), respectively.





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