QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 8. GNL3L suppresses SRC-mediated transcriptional coactivation of ERR ${gamma}$ . (A) Using the same cell-based reporter system as described in Fig. 4, we show that the ERE-specific transcriptional activity in cells coexpressing ERR ${gamma}$ and SRC1 (8.0±0.3) is 1.7 times higher than that of the ERR ${gamma}$ -expressing sample (4.8±0.3). When coexpressed with the wild-type GNL3L (WT), this ERR ${gamma}$ and SRC1-mediated ERE-specific transcriptional activity is reduced by 55% and 70% compared with the sample expressing both ERR ${gamma}$ and SRC1 in a dose-dependent manner. This inhibitory effect of GNL3L on the SRC1-mediated coactivation of ERR ${gamma}$ requires the I-domain of GNL3L because deletion of this domain (dI) fails to suppress the transcriptional activity of ERR ${gamma}$ and SRC1 (P=0.17). (B) Using the same approach, we show that GNL3L can also suppress the coactivator function of SRC2 on the ERR ${gamma}$ -dependent transcriptional activity in a dose-dependent (54% reduction for 100 ng of GNL3L and 71% reduction for 200 ng of GNL3L) and I-domain-dependent (P=0.58) manner. Error bars represent the stand error of mean (± s.e.m.). ^***P<0.0001.

Return to article