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Figure 2


Fig. 2. MDCK cells expressing Fc38-63 or the human transferrin receptor were transfected with EGFP-tagged Eps15{Delta}95-295 (B,E). The cells were incubated with Alexa Fluor-594-transferrin (A) or anti-Fc receptor antibodies (D) for 30 minutes at 4°C and then shifted to 37°C for 30 minutes. Cells were then fixed and immunofluorescent molecules were directly visualized (A-C) or the cells were fixed, permeabilized and incubated with anti-rat IgG conjugated to Alexa Fluor-594 prior to microscopic analysis (D-F). Bars, 10 µm.





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