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Figure 6


Fig. 6. Polarized MDCK cells expressing Fc38-63 were incubated in the presence of 10 mM methyl-beta-cyclodextrin for 1 hour at 37°C. The cells were then fixed, permeabilized and incubated with antibodies specific for the Fc receptor (A) and caveolin 1 (B). Alternatively, untreated cells were incubated with anti-Fc receptor antibodies (D-F) for 30 minutes at 4°C. The cells were then washed, fixed, permeabilized and incubated with antibodies specific for caveolin 1 (E). In both instances, the cells were washed and incubated with anti-rat IgG conjugated to Alexa Fluor-594 (A,D) and anti-rabbit IgG conjugated to Alexa Fluor-488 (B,E). Bars, 10 µm.





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