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Figure 3


Fig. 3. PIASxbeta overexpression increased ALP activity and ALP, Osx and OCN mRNA levels. (A) MC3T3-E1 cells were transfected at 90% confluence with the indicated expression plasmids and subjected to ALP staining at days 6 and 9 in DM. (B) Cells were transfected at day 6 in DM with the indicated expression plasmids and subjected to ALP staining at day 10. (C) Cells were transfected with the expression plasmids at day 6 in DM and RNA samples were collected at day 9 for RT-PCR analysis. (D) mRNA expression level in C was also performed by quantitative real-time RT-PCR.





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