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Figure 2


Fig. 2. Mapping the binding sites on T6BP, NDP52 and myosin VI. (A) The zinc fingers of T6BP bind to myosin VI. Expression plasmids encoding myosin VI tail constructs were used as bait and either T6BP truncation constructs or site-directed mutagenesis constructs as prey. (B) The LIM-like domain of NDP52 binds to myosin VI. Expression plasmids used were as in A but as prey either NDP52 truncation constructs or site-directed mutagenesis constructs were used. (C) T6BP and NDP52 cartoons showing the N-terminal SKICH domains, shaded boxes predicting regions of {alpha}-helical coiled coil and the zinc fingers (Z). (D) T6BP and NDP52 interact with the RRL motif in the myosin VI tail. Expression plasmids used were as in A except that those encoding myosin VI wild type (WT) and mutant (RRL to AAA) tail constructs were the bait and T6BP or NDP52 constructs were the prey.





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