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Figure 3


Fig. 3. Intracellular localisation of T6BP and NDP52 in HeLa cells. (A) T6BP is localised at the Golgi complex and in the perinuclear region of the cell using anti-T6BP antibody (a and b) or by GFP-T6BP expression (c). Cells in d-f were pre-permeabilised with saponin and double labelled with antibody against TGN46 (e and e'), the box area in panel f is shown enlarged in panels d'-f'. Cells in panels g-i were double labelled with antibodies against T6BP and vinculin. (B) NDP52 is localised at the Golgi complex and in the perinuclear region of the cell by anti-NDP52 antibodies (a and b) or by GFP-NDP52 expression (c). To localise NDP52 around the Golgi complex, cells in panels d-f were pre-permeabilised with saponin and double labelled with TGN46 antibodies (e and e'). The boxed area in panel f is shown enlarged in panels d'-f'. Bars, 10 µm.





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