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Figure 1


Fig. 1. Location of the T-DNA inserts in Arabidopsis CAP1 (At4g34490). Translated sections of exons of Arabidopsis CAP1 are represented by boxes, and introns are represented by horizontal lines. T-DNAs are not drawn to scale. Primer 1 (CAP28F) combined with primer 2 (CAP28R) was capable of amplifying CAP1 cDNA from azygous plants but not from cap1-1 or cap1-2 homozygote plants (see Fig. 2). Products could be amplified with primers 1 and 2 combined with T-DNA primers (3 and 4, respectively) using the appropriate homozygote plant genomic template, but not from a cDNA template, which suggests the absence of processed CAP1:T-DNA fusion transcripts in homozygote mutant plants.





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