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Fig. 2. RT-PCR shows that CAP1 is absent in plants homozygous for alleles cap1-1 and cap1-2 (A). The full-length CAP1 transcript can be amplified from plants with a wild-type CAP1 allele (1), but not from plants homozygous for either cap1-1 or cap1-2 (2). Control individuals are azygous plants from populations segregating cap1-1 or cap1-2. Primer combinations are illustrated in Fig. 1. All plants were successful templates for amplifying the control GAPC transcript (upper gel). Plants homozygous for cap1 alleles show growth deficiencies when compared with wild-types (B), the most obvious of which is a reduction in the rate of inflorescence development (plants were photographed at 53 DAG; bar, 5 cm). Epidermal peels taken from primary inflorescences between the second and third developing silique at 42 DAG demonstrate that wild-type cells (C) are further elongated than equivalent cap1 cells (D). Individual primary inflorescences chosen for comparison bore equal numbers of mature lateral organs. Wild-type GFP:FABD2 primary inflorescence epidermis cells (E) have a parallel arrangement of fine actin cables along the axis of cell expansion. Mutant epidermis (F) contains shorter F-actin bundles poorly aligned with respect to the axis of growth. Bars, 200 µm.

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