|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 4. The in vitro growth behaviour of pollen grains of different genotypes was confirmed in vivo. Flowers were pollinated and after incubation were dissected and stained with aniline blue. After 4 hours (left panel) large numbers of wild-type pollen grains have germinated and produced intensely staining callose deposits. Many wild-type pollen tubes have grown the length of the style tissue and are beginning to enter the ovary. A minority of cap1 pollen grains show signs of germination at this time point, and only auto-fluorescence from vascular tissue is visible within the style. Stigmas stained at 5.5 hours (centre panel) after pollination with wild-type pollen contain a significant number of pollen tubes developing callose plugs (white arrows) within the ovary, and some tubes are contacting ovules. A greater proportion of mutant pollen grains are germinating but tube growth is still retarded. Stigmas stained after 24 hours of pollen tube growth (right panel) shows that some mutant pollen tubes do eventually contact ovules. Bars, 200 µm.
|
