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Figure 5


Fig. 5. Effects on global histone acetylation patterns of COS-7 cells transfected with Aiolos isoforms. (A) Western blot analysis of histone modifications of cell extracts. Analysis with antibodies against acetyl-H4, acetyl K16 and K8 of H4, acetyl-H3 and acetyl K9 of histone H3. A Coomassie Blue staining is also shown as a loading control. (B) Analysis of histone H4 acetylation by high performance capillary electrophoresis (HPCE). An example of HPCE separation of acetylated species of histone H4 from pcDNA3 and Aiolos-transfected COS-7 cells. The non-acetylated (Ac0), monoacetylated (Ac1) and diacetylated (Ac2) forms of histone H4 are indicated. (C) A quantification of the HPCE analysis showing the acetylation of histone H4 corresponding to COS-7 cells transfected with all Aiolos isoforms.





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