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Figure 4


Fig. 4. Xevl is required for cellular adhesion. (A) Confocal image of a whole-mount embryo at stage 35, showing Xevl immunostaining in the otic vesicle; a higher magnification image shown in A'. Xevl is enriched apically throughout the otic vesicle epithelium (arrow) and at cellular adhesions. Xevl is also highly expressed in the ventromedial sensory epithelium where mechanosensory hair cells form (arrowhead). (B) Dissociated otic vesicles from uninjected stage 35 embryos form large aggregates. (C) By contrast, otic vesicle cells from XevlMO-injected embryos form smaller and more loosely adherent aggregates. Insets in B and C show higher magnification of the aggregates indicated by dashed boxes. Bars, 50 µm.





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