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Figure 5


Fig. 5. Xevl localizes to sensory structures of the inner ear. Confocal imaging of frozen sections of stage 35 embryos. Dorsal is up and the otic vesicle is to the right in all images. (A) Xevl is present in the vestibulocochlear ganglion (arrow) as well as in cells within the otic vesicle sensory epithelium (arrowhead). (B) Islet-1 localization in the vestibulocochlear ganglion (arrow) and within the otic vesicle sensory epithelium (bracket). (C) The merged image reveals Xevl (red) is more strongly expressed in the cells that also have strong islet-1 staining (green). DAPI staining is in blue. (D-F) Xevl (D, red in F) colocalizes with neural neurofilament (nf; E, green in F) in neurons of the vestibulocochlear ganglion and the neurites that extend to innervate the otic vesicle epithelium (arrowhead in F) and the hindbrain (arrow in F). Bar, 20 µm.





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