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Figure 6


Fig. 6. Ectopic Abcg2 expression adversely affects differentiation of late retinal progenitors. (A-M). There was a significant decrease and increase in the proportion of Map2+ (A-D,M)/GFAP+ (E-H,M) and Nestin+ (I-L,M) cells, respectively, in G1-ABCG2 retrovirus-transduced neurospheres, compared with controls. (N) RT-PCR analysis revealed a decrease and increase in levels of transcripts corresponding to Map2/GFAP and Nestin, respectively, in G1-ABCG2-transduced neurospheres, compared with controls, corroborating the immunocytochemical analysis. (O) RT-PCR analysis revealed a decrease in the expression of transcripts encoding the specific retinal cell markers, rhodopsin kinase (RK, rods), mGluR6 (bipolar cells), Syntaxin1 (amacrine cells) and glutamine synthetase (GS, Müller cells) in G1-ABCG2 transduced neurospheres compared with controls, demonstrating the effects of ABCG2 on retinal-cell-specific differentiation. Data are expressed as the mean ± s.e.m. of triplicate cultures from two different experiments. ***P<0.005 compared with levels in the control. Magnification, 200x.





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