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Figure 1


Fig. 1. Depletion of PrPC by RNAi. (A) Western blot of PrPC levels in control (Mock) SN56 cells and cells 48 hours after being transfected with the following oligonucleotides: scramble sequence (Scram), sequence 1, or sequence 2. In each lane, 100 µg of total protein was loaded. (B) Quantification of the western blot shown in A. The different intensities of the PrPC bands were normalized to the mock-depleted value which was set at 100%. Quantification of the western blot showed that it was linear from 10 to 100 µg of cell lysate. (C) Immunoprecipitation of PrPC from 500 µg cell lysates obtained from control cells and cells transfected with the scramble sequence, sequence 1, or sequence 2. The control cells were treated with Lipofectamine, the same as the PrPC-depleted cells.





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