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Figure 4


Fig. 4. Shear flow negatively regulates the level and localization of activated beta-catenin. Localization of cyclin D1 in control cells (A) and cells exposed to shear stress (B). Localization of beta-catenin in control cells (C) and cells exposed to shear stress (D). Localization of dephosphorylated activated beta-catenin in control cells (E) and cells exposed to shear stress (F). Lanes 1-4 of the inset in F show a western blot of dephosphorylated beta-catenin in the cytoplasmic (lanes 1, 3) and NP-40 pools (lanes 2, 4) before (lanes 1, 2) and after (lanes 3, 4) shear stress. The numbers above the lanes indicate the densitometric ratio of activated beta-catenin (static:shear).





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